Isolation and characterization of antibacterial endophytic bacteria. For the isolation of endophytic bacteria, the tissue extract was diluted in an aqueous solution 0. Isolation and identification of indigenous endophytic bacteria. Isolation procedures for endophytic microorganisms. Alkalibacillus was used as an outgroup which derived from the same family with bacillus. Screening of endophytic bacteria isolated from leaves of. May 30, 2017 isolation and identification of endophytic bacteria with plant growth promoting and biocontrol potential from oak trees. We successfully isolated endophytic bacteria from six out of 10 eucalyptus species and studied hybrids. The plant material used in this study was the above ground parts of chelidonium majus plants collected at the flowering stage between isolation and characterization of bacterial endophytes of chelidonium. Screening, isolation, biochemical and plant growth promoting. From 51 sugarcane samples of 7 districts sites ben cau. Isolation and molecular identification of endophytic.
Isolation of endophytic bacteria the surfacedisinfected plant organs roots, stems, and leaves were removed with a sterilized razor blade, and the organs were cut into pieces 1 3 mmlong, which were placed on tsa plates. Isolation and identification of endophytic bacteria with. Five different taxa were represented by 11 isolates, and six isolates were. Isolation of putative endophytic diazotropic bacteria for p solubilisation endophytic bacteria were isolated from different parts of selected rice plant such as root and employing standard microbiological methods. Pdf isolation and identification of fungal endophytes. Endophytic bacteria colonize a large number of plants, which include plant growthpromoting bacteria. Endophytic bacteria form associations with plants, at least in one phase in their life cycle. Endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. Isolation and characterisation of some endophytic bacteria. Isolation and identification of endophytic fungi from.
Based on the culturedependent method, a total of 165 cultivable endophytic bacteria isolates were isolated from the sterilized dendrobium. Isolation, characterization, endophytic bacteria, antibacterial, mangrove. Isolation of endophytic bacteria from experimentally inoculated plants. Zinniel dk, lambrecht p, harris nb, feng z, kuczmarski d, higley p, ishimaru ca, arunakumari a, barletta rg, vidaver ak 2002 isolation and characterisation of endophytic colonising bacteria from agronomic crops and prairie plants. Berg and hallmann6 confirmed that a significant portion of the indigenous endophytic bacteria in plant roots have antagonistic potential toward fungal pathogens, but little is known about the fluctuation and structure of the aeb population. Frontiers isolation, diversity, and growthpromoting. Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties. The isolation, identification and screening of endophytic bacteria for the plant growth regulators like cytokinin are needed to get the leads for their applications in agriculture sector. Isolation of endophytic fungi from zygophyllum mandavillei. A novel method to scale up fungal endophyte isolations melinda greenfielda, reynaldo parejaa, viviana ortiza, maria i. In our study, we isolates four endophytic bacteria from explants root,stem, petiole, and leaf of aloe barbadensis, adhatoda vasica, phyllanthus amarus and calotrophis. Jun 21, 2012 chemical isolation and characterization of active compound. Nov 01, 2010 endophytic bacteria are harmless in most plant species. The present work was aimed to isolate the endophytic bacteria from different medicinal plants such as aloe barbadensis,adhatoda vasica,phyllanthus amarus.
Vegab and soroush parsaa aentomology, international center for tropical agriculture ciat, cali, colombia. Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment. Biocontrol agents, endophytic bacteria, plantgrowthpromoting traits. The plates were examined for growth after incubation at 288c for 3 days. For instance, application of glyphosate herbicide is common mainly due to the use of glyphosateresistant transgenic plants.
Isolation of endophytic fungi from thermopsis lanceolata and their antioxidant activity lin yang1, xiaokai zhou1, lei wang1, hongxia shi2, xiaofeng liu1, yonggang wang1 1the key lab of screening, evaluation and advanced processing of tcm and tibetan medicine, gansu educational department. Endophytic bacteria in agricultural crops article pdf available in canadian journal of microbiology 4310. Isolation, identification and characterisation of endophytic. In addition, speculative conclusions are raised on some points to stimulate thought and research on endophytic bacteria. Abstract endophytic bacteriareside with in plant hosts without causing disease.
Following incubation, cell free culture medium was concentrated under vacuum to reduce the volume to approximately 800 ml. Mundt and hinkle, 1976 more than 200 bacterial genera from 16 phyla have been reported as endophytes. Isolation, identification and screening of endophytic. Isolation and molecular identification of endophytic bacteria from rambutan fruits nephelium.
The fungus cladosporium cladosporioides was the predominant isolated fungus with colonization and dominance frequencies percentage 12. Isolation and characterization of endophytic bacteria from. Endophytic fungi from the chinese medicinal plant actinidia macrosperma were isolated and identified for the first time. Isolation and characterization of endophytic colonizing. Medium and main reagents endophytic bacteria were isolated and cultured in na culture media containing 3 g beef extract, 5 g peptone, 5 g nacl, 18 g agar, and ml water with ph 7. A novel method to scale up fungal endophyte isolations. Many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more. Isolation and characterization of antibacterial compound from. Bacteria isolation, colony characteristic and microscopic examination the endophytic bacteria developed in the pellicles of semi solid in lgi medium as the previous results of weber et al. Pdf endophytic bacteria are ubiquitous in most plant species, residing latently or actively colonizing plant tissues locally as well. Jul 17, 2019 diversity of endophytic bacteria based on illuminabased analysis quality control and analysis of sequencing data. To our knowledge, the present work is the first report on the isolation, identification and enzymatic characterization of endophytic microorganisms from r.
A total of 7, 422 reads and 655 otus were obtained after clustering at a 97%. Diversity of cultureindependent bacteria and antimicrobial. This endophytic bacterial community may be affected by crop management such as the use. Isolation and characterization of endophytic bacteria from plectranthus tenuiflorus medicinal plant in saudi arabia desert and their antimicrobial activities. Isolation and characterization of endophytic colonizing bacteria. Pdf endophytic bacterial isolates were isolated from roots, nodules, leaves and stems of faba bean vicia faba, pea pisum. Pdf endophytic bacteria and their potential applications. View endophytic bacteria research papers on academia. Isolation and screening of p solubilising endophytic. Isolation of endophytic bacteria from rambutan fruit in sap produces nine isolates which were. Isolation, identification and screening of endophytic bacteria antagonistic to biofilm formers hira muzzamal, rabbia sarwar, imran sajid and shahida hasnain department of microbiology and molecular genetics, university of the punjab, quaideazam campus, lahore 54590, pakistan abstract. Omonrice 12 2004 95 the two hosts are given in table 1. This endophytic bacterial community may be affected by crop management such as the use of chemical compounds. Isolation and pcr amplification of the v3v4 16s rdna region of endophytic bacterial isolates from saccharum sp a saccharum sp from jamaica used for the isolation of endophytic bacteria.
Isolation and enzyme bioprospection of endophytic bacteria. University of groningen bacterial endophytes of rice hardoim. Isolation of antagonistic endophytes from banana roots against meloidogyne javanica and their effects on soil. Jul 28, 2014 however, studies focusing on the isolation and characterization of endophytic bacteria from native plant specimens from the brazilian mangrove biome are rare. Rhizome of ginger zingiber officinale was collected from navajyothisree karunakara guru research centre for ayurveda and siddha, uzhavoor, kottayam and was used as the source material for the isolation of endophytic bacteria. Yan x, wang z, mei y, wang l, wang x, xu q, peng s, zhou y and wei c 2018 isolation, diversity, and growthpromoting activities of endophytic bacteria from tea cultivars of zijuan and yunkang10. Antagonistic endophytic bacteria aeb are promising agents for biological control agents. Endophytic bacteria are ubiquitous in most plant species influencing the host fitness by disease suppression, contaminant degradation, and plant growth promotion. Isolation and genomic characterization of six endophytic. Screening, isolation, biochemical and plant growth promoting characterization of endophytic bacteria. Endophytic bacteria normally live on intercellular spaces that contain carbohydrates, amino acids, and high amounts of inorganic nutrients bacon and. Pdf isolation and characterization of endophytic bacteria isolated. Diversity of cultureindependent bacteria and antimicrobial activity of. Furthermore, the isolation of endophytic bacteria from withania coagulans and olea ferruginea is novel in association to these plants.
Downloaded from the university of groningenumcg research database pure. Since the first reliable reports about the isolation of endophytic bacteria from surfacesterilized plants samish et al. Abid ullah 1, hafsa mushtaq 2, usman ali 1, hakim 1, ehsan ali 3, samavia mubeen2. Endophytic bacteria with plant growth promotion and. B plating and isolation of bacteria on tryptic soy agar tsa, luria broth agar lb, reasoners 2a agar r2a, nutrient agar n and potato dextrose agar. All solvents used for extractions, isolation and purification were of hplc grade. Owing to these characters, the representative isolated bacteria are suggested to be used in biofertilizer and biopesticides. Bioactivities of bacterial endophytes isolated from leaf tissues of.
This was the first study to evaluate their cytotoxic and antitumour activities against brine shrimp and five types of tumour cells, respectively. Bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in brazil. An array of 76 endophytic bacterial isolates obtained. Diversity of endophytic bacteria from eucalyptus species. Putative endophytic bacterial strains were defined as isolates that were obtained from surfacesterilized plants, displayed differentiable colony morphologies, and were recovered from the initial survey of agronomic crops and prairie grasses. Isolation and molecular identification of endophytic bacteria. Isolation of antagonistic endophytes from banana roots. Diversity of cultivated endophytic bacteria from sugarcane. Isolation and characterization of bacterial endophytes of. Isolation of potential antimicrobial metabolites from. Isolation and molecular identification of endophytic bacteria from.
Bacterial endophytes in agricultural crops canadian journal. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Plating of triturated seed samples showed that total cultured bacterial densities were significantly different p. Isolation and characterization of endophytic bacteria in soybean glycine sp. Ruan y, tao c, chao y, li r and shen q 2017 isolation of antagonistic endophytes from banana roots against meloidogyne javanica and their effects on soil nematode. The isolation of the endophytic bacteria was conducted within 48 h of the fieldwork. Isolation is done to obtain pure bacterial cultures. These include both culturable and unculturable bacteria belonging to acidobacteria, actinobacteria. Isolation of endophytic bacteria from dendrobium stems.